View Project Rogers-3U24NS043571-01S1

Project Summary
Status:	Public
Publications:	None
Project Detail		Data Detail
Platform:	Affymetrix	MIAME Areas	Compliance
Species:	Human	Array Design Detail	true
Organ/Tissue Type:	blood	Experiment Detail	false
Organ Region:	adrenal medulla	Sample Detail	false
Cell Type:	chromaphine cells	Hybridization Detail	false
Study Type:	subclassification	Measurement Detail	false
Disease/Condition:	Aged, Young control
Replicates:	0
Expected Samples:

Available Actions

Investigator Contact Detail
Name	Joseph Rogers
Street Address:	Sun Health Research Institute
	10515 W Sante Fe Drive
City, State/Province:	Sun City , AZ
Zip/Postal Code:	85372
Country:	United States
Work Phone:	623-876-5328
Fax:
E-mail:	sbrautigam@tgen.org

Proposal Detail

Grant:

3U24NS043571-01S1

Status:

Public

Service Type:

Start to Finish Profiling

IRB:

1234

IRB date:

2003-07-24

Study Relevance:

Alzheimer's Disease (AD) is a devastating neurodegenerative disorder affecting approximately 4 million people in the U.S. alone. AD is characterized by the presence of senile plaques and neurofibrillary tangles in cortical regions of the brain. These pathological markers are thought to be responsible for the massive cortical neurodegeneration and concomitant loss of memory, reasoning, and often aberrant behaviors that are seen in patients with AD. Understanding the molecular mechanisms whereby these histopathological markers develop will greatly enhance our understanding of AD development and progression. A clearer understanding of the mechanisms underlying neurofibrillary tangle formation specifically may help to clarify the basis for dementia of AD as well as the dementias associated with other diseases that are collectively referred to as "tauopathies."

Hypothesis:

The gene expression profile of neurons that contain neurofibrillary tangles will differ from the expression profile of histopathologically normal neurons from the same patient and from the same brain region. Some of these differences will be informative as to the mechanisms of tangle formation.

Specific Aim:

To expression profile both neurons containing neurofibrillary tangles and normal neurons from the entorhinal cortex of 10 mid-stage AD cases.

Experimental Procedure and Design:

First use laser capture microdissection to select 1000 neurons bearing neurofibrillary tangles and 1000 normal neurons from the layer 2 stellate island neurons of the entorhinal cortex of 10 mid-stage AD cases. Second, to isolate the RNA from these captured neurons, perform double round linear amplification and hybridize the labeled cRNA to affymetrix U133A arrays. Third, to use paired, permutational t-tests to analyze the microarray data to select tangle-specific differences in gene expression. For the purposes of submitting this proposal, a value of 1 ug of RNA was entered due to web site constraints on values placed in that field. However, all samples are from LCM captured cells and the actual RNA yield is likely closer to 100 pg.

Experimental Factors:

Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.


Experimental Factors is empty.

Project Samples

Samples associated with this project.

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Name	Description	Bio-Source	Extracts
EC_00-31 control	entorhinal cortex layer II ...	Rogers	1
EC_00-31 tangle	entorhinal cortex layer II ...	Rogers	1
EC_00-34 control	entorhinal cortex layer II ...	Rogers	1
EC_00-34 tangle	entorhinal cortex layer II ...	Rogers	1
EC_00-36 control	entorhinal cortex layer II ...	Rogers	1
EC_00-36 tangle	entorhinal cortex layer II ...	Rogers	1
EC_01-30 control	entorhinal cortex layer II ...	Rogers	1
EC_01-30 tangle	entorhinal cortex layer II ...	Rogers	1
EC_102-30_control	entorhinal cortex layer II ...	Rogers	1
EC_102-30_tangle	entorhinal cortex layer II ...	Rogers	1
EC_102-37_control	entorhinal cortex layer II ...	Rogers	1
EC_102-37_tangle	entorhinal cortex layer II ...	Rogers	1
EC_582_control	entorhinal cortex layer II ...	Rogers	1
EC_582_tangle	entorhinal cortex layer II ...	Rogers	1
EC_725_control	entorhinal cortex layer II ...	Rogers	1
EC_725_tangle	entorhinal cortex layer II ...	Rogers	1
EC_98-20 control	entorhinal cortex layer II ...	Rogers	1
EC_98-20 tangle	entorhinal cortex layer II ...	Rogers	1
EC_98-21 control	entorhinal cortex layer II ...	Rogers	1
EC_98-21 tangle	entorhinal cortex layer II ...	Rogers	1

Project Hybridizations

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Name	Array	Labeled Extract
Hybridization20	Human Genome U133 Plus 2.0 Array_1	00 28 normal_e1_le1
Hybridization21	Human Genome U133 Plus 2.0 Array_2	00-28 tangle_e1_le1
Hybridization22	Human Genome U133 Plus 2.0 Array_3	01-22 normal_e1_le1
Hybridization23	Human Genome U133 Plus 2.0 Array_4	01-22 tangle_e1_le1
Hybridization24	Human Genome U133 Plus 2.0 Array_5	95-08 normal_e1_le1
Hybridization25	Human Genome U133 Plus 2.0 Array_6	95-08 tangle_e1_le1
Hybridization26	Human Genome U133 Plus 2.0 Array_7	96-18 normal_e1_le1
Hybridization27	Human Genome U133 Plus 2.0 Array_8	96-18 tangle_e1_le1
Hybridization28	Human Genome U133 Plus 2.0 Array_9	96-31 normal_e1_le1
Hybridization29	Human Genome U133 Plus 2.0 Array_10	96-31 tangle_e1_le1
Hybridization30	Human Genome U133 Plus 2.0 Array_11	97-01 normal_e1_le1
Hybridization31	Human Genome U133 Plus 2.0 Array_12	97-01 tangle_e1_le1
Hybridization32	Human Genome U133 Plus 2.0 Array_13	98-18 normal_e1_le1
Hybridization33	Human Genome U133 Plus 2.0 Array_14	98-18 tangle_e1_le1
Hybridization34	Human Genome U133 Plus 2.0 Array_15	99-21 normal_e1_le1
Hybridization35	Human Genome U133 Plus 2.0 Array_16	99-21 tangle_e1_le1
Hybridization36	Human Genome U133 Plus 2.0 Array_17	99-28 normal_e1_le1
Hybridization37	Human Genome U133 Plus 2.0 Array_18	99-28 tangle_e1_le1
Hybridization38	Human Genome U133 Plus 2.0 Array_19	99-33 normal_e1_le1
Hybridization39	Human Genome U133 Plus 2.0 Array_20	99-33 tangle_e1_le1

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